Capability of core-sheath polyvinyl alcohol- Polycaprolactone emulsion electrospun nanofibrous scaffolds in releasing strontium ranelate for bone regeneration

Core-sheath nanofibrous scaffolds from polyvinyl alcohol (PVA)-strontium ranelate (SrR)- Polycaprolactone (PCL) were prepared by water in oil electrospinning method. Thus, PCL (the oil phase) was used as the shell part and a mixture of PVA and SrR (the water phase) was inserted in the core. The amounts of SrR was varied from 0 to 15wt.% Mussel-inspired dopamine-gelatin coating was done on the nanofibrous to improve their hydrophilicity and cellular attachment. The effect of the SrR content on morphology, mechanical, physicochemical, in vitro release behaviors, and biological properties as well as in vivo bone regeneration was investigated. Morphological observations revealed that continuous nanofibers with a core/shell structure were successfully obtained and the fibers diameter increased as the SrR content rose. X-ray diffraction (XRD) analysis revealed that SrR was molecularly distributed in the nanofibers and increasing the amount of the SrR decreased the crystallinity of the nanofibers. Moreover, the SrR release was regulated through the mechanism of Fickian diffusion and it was assumed as fast as possible in the samples with higher SrR content. The mesenchymal stem cell culturing showed improved cell proliferation by adding SrR and accelerating the expression of ALP, Runx2, Col I, and OCN genes. Besides, the SrR-loaded nanofibers improved bone formation of calvarial defects in a rat model as revealed by in vivo investigations.

Seyyed Behnam Abdollahi Boraei,Jhamak Nourmohammadi,Behnaz Bakhshandeh,Mohammad Mehdi Dehghan,Hossein Gholami,Zoilo Gonzalez,Antonio Javier Sanchez-Herencia,Begona Ferrari

Seyyed Behnam Abdollahi Boraei, Institute of Ceramics and Glass, Ceramic and Glass Institute – CSIC. C / Kelsen 5. Cantoblanco Campus, Madrid, 28049, SPAIN.

Jhamak Nourmohammadi, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran, Faculty of New Science and Technology, North Kargar Street, Tehran, Iran, Tehran, 1439957131, Iran (the Islamic Republic of).

Behnaz Bakhshandeh, Department of Biotechnology, College of Science, , University of Tehran, Tehran, Iran , College of Science, University of Tehran, Enghelab Avenue, Tehran, Iran, Tehran, 141556455, Iran (the Islamic Republic of).

Mohammad Mehdi Dehghan, University of Tehran, Faculty of Veterinary Medicine, University of Tehran, corner of Dr. Gharib St., Azadi St., Tehran, Tehran, 1419963111 , Iran (the Islamic Republic of).

Hossein Gholami, Institute of Biomedical Research, University of Tehran Faculty of Veterinary Medicine, Faculty of Veterinary Medicine, University of Tehran, corner of Dr. Gharib St., Azadi St., Tehran, Tehran, Tehran, 1419963111, Iran (the Islamic Republic of).

Zoilo Gonzalez, Institute of Ceramics and Glass, Ceramic and Glass Institute – CSIC. C / Kelsen 5. Cantoblanco Campus. 28049 Madrid, Madrid, 28049, SPAIN.

Antonio Javier Sanchez-Herencia, Institute of Ceramics and Glass, Ceramic and Glass Institute – CSIC. C / Kelsen 5. Cantoblanco Campus, Madrid, 28049, SPAIN.

Begona Ferrari, Instituto de Ceramica y Vidrio, Consejo Superior de Investigaciones Cientificas, Ceramic and Glass Institute – CSIC. C / Kelsen 5. Cantoblanco Campus, Madrid, 28049, SPAIN.

Courtesy of Physician's Weekly

https://www.physiciansweekly.com/capability-of-core-sheath-polyvinyl-alcohol-polycaprolactone-emulsion-electrospun-nanofibrous-scaffolds-in-releasing-strontium-ranelate-for-bone-regeneration/